Directions for collecting and sending samples

Metabolic investigation

Amniotic fluid supernatant

The sample must be collected in the 16th - 18th week of gestation. After the removal of cells by centrifugation, the amniotic fluid supernatant is stored at -20°C and sent frozen on dry-ice. Please contact Dr. Mirjam Wamelink prior to sending amniotic fluid.

Plasma and serum

In general heparinized plasma is preferred to EDTA plasma, citrate plasma, fluoride plasma or serum. Only for the assay of homocysteine EDTA plasma is preferred. After collection, the blood must be centrifuged as soon as possible for 10 minutes at 1800 g. The plasma or serum is stored and sent at -20°C.

Urine

A random urine sample (minimum of 10 ml required) can be sent. The urine is stored and sent at  -20°C. In special cases urine can be spotted on filter-paper, dried and send to our laboratory by regular mail. Please contact Dr. Mirjam Wamelink prior to sending samples.

Deproteinated CSF or blood for lactate, pyruvate and ketones

See Lactate, pyruvate and ketones protocol, this test is performed at location AMC.

CSF 

Analysis of neurotransmitters and other classes of compounds in CSF have specific sampling conditions, see our CSF protocol .

Moleculair studies  

Blood and DNA 

2 ml EDTA blood or isolated DNA can be sent at room temperature. In the case that an email address is provided we will send a confirmation upon arrival of the package. Please contact  Prof. Gajja Salomons for instructions about molecular prenatal diagnosis.

Directions for sending samples 

Samples must be sent on dry-ice (except for cultured cells or blood for DNA isolation). Make sure the samples will be kept frozen for a period of 48 hours. Please use our request forms and provide the necessary information like patient data, requesting physician and sample information.

If you have any questions or you require additional information, do not hesitate to contact Dr. Mirjam Wamelink or Prof. Gajja Salomons .

The parcel should be sent in the beginning of the week (i.e. Monday to Wednesday) in order to reduce the risk that samples will arrive in the weekend.

Shipping address:

VU University Medical Center 
Metabolic Laboratory (PK 1X 009)
To: Prof. G.S. Salomons
Goederenontvangst - BS6
Van der Boechorststraat 6
1081 BT Amsterdam
The Netherlands