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Coenzyme A esters

In the mitochondrial ß-oxidation cycle, the conversion of straight-chain acyl-CoAs into 2-trans-enoyl-CoAs is catalyzed by acyl-CoA dehydrogenases, differing in chain length specificities. Phenylpropionyl-CoA is specificly accepted by Medium-Chain Acyl-CoA Dehydrogenase yielding cinnamoyl-CoA as product. In a variety of cell types MCAD can be assayed spectrophotometrically by measuring at 308 nm the formation of cinnamoyl-CoA from phenylpropionyl-CoA in the presence of an artificial electron acceptor.

Standards
- 3-phenylpropionyl-CoA
- cinnamoyl-CoA

Application
Yao K-W, Schulz H. Specific assay of Medium-Chain Acyl-CoA Dehydrogenase based on the spectrophotometric maesurement of product formation. Anal Biochem 214 (1993) 528-534.

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