For isolation of high quality DNA orRNA we recommend the protocols on this website. The following conditions are required:
The DNA or RNA should be suspended in nuclease free water and absolutely free from organic or any other contaminations like chelating agents or salt.
Acceptable ratios for genomic DNA are (measured with a NanoDrop):
260/280: a ratio of ~1.8
260/230: a ratio of 1.8-2.2
If these ratios are lower, an additional purification is necessary.
For Agilent arrays at least 500 ng of DNA in 5 ul is needed. The NimbleGen arrays require at least 1000g of DNA in 10 ul. The DNA must be double stranded.
For both platforms (Agilent and NimbleGen) it is possible to use DNA isolated from FFPE material. To obtain the best results from DNA isolation from FFPE, please use our protocols on our website.
If your genomic DNA may contain inhibitors, our cleanup protocols can be used.
For RNA micro array analysis, we require high quality total RNA. For isolation we recommend the protocols on our website. The following conditions are required:
Acceptable ratios for RNA are (measured with a NanoDrop):
260/280: a ratio of >1.8
260/230: a ratio of >1.8
For each array routinely 500 ng of RNA in 10 ul is needed. The RNA must be total RNA.
The RNA integrity has to be checked with the BioAnalyzer.
Provide aliquots of approximately 5 ul RNA of about 200 ng/ul, diluted in water.